Is There an Influence of Electrically Stimulated Osteoblasts on the Induction of Osteoclastogenesis?

Bone is a highly dynamic tissue characterized mainly by the interactions of osteoblasts and osteoclasts. When healing ability bone regeneration disturbed, targeted biophysical stimulations such as electrical stimulation are applied. In this study indirect effects electrically stimulated human on osteoclastogenesis were investigated to better understand detailed cellular interactions. Therefore, two different cell developmental stages examined: peripheral blood mononuclear cells (PBMCs) precursors pre-osteoclasts differentiated cells. Previously, over 21-day period, with low-frequency alternating electric field. The supernatants collected used for an co-culture PBMCs pre-osteoclasts. viability induction differentiation activity analyzed. Further, secretion relevant osteoclastic markers was examined. Supernatants 7 d 14 led decrease in increased number containing actin ring structures. from induced PBMC pre-osteoclastic activation. Furthermore, showed varying mRNA transcripts MCP-1, ACP5, CA2, CASP8 when cultivated media osteoblasts. day 21 did not influence at all but We could show that time points have changes can be observed due Through stimulation, it possible underline importance studying only osteoblastic mineralization behavior under also analyzing